اثر درمان با پالس‌الکترومغناطیس بر ریز‌ساختارهای بافت استخوان ران در موش‌های آزمایشگاهی اواریکتومی‌شده

Background and Aim: Osteoporosis is a metabolic bone disease that can result from cytokines activity such as TNFɑ. The aim of this study was to evaluate the effect of pulsed electromagnet therapy on&nbsp; femoral strength and bone microstructure in ovariectomized rats. Materials and Methods: In this experimental study, 30 rats were randomly divided into control, experimental1 (ovariectomized) and two experimental groups; namely ovariectomized and undergoing pulsed electromagnet groups. &nbsp;The control and experimental1 groups were kept under controlled conditions, while the two experimental groups were treated with pulsed electromagnet (2.4 mT) from 12 postoperative weeks for 30min, 3days a week, for 10 weeks. Then, the subjects were sacrificed and their femoral bones were removed to determine the strength and the bone microstructure&nbsp; parameters (the trabecular and cortical thicknesses and trabecular distances). In order to determine bone microstructures, the sections were prepared and stained with H&E. Then, Haworth method was used to measure. One-way ANOVA, repeated measurements, and Scheffe post- hoc tests were applied to analyze the obtained data. Statistical analyses were performed using SPSS software (version 16; Chicago,IL). Results: Despite equal initial weight of the subjects (P=0.15), they significantly gained weight after12 and also 22 postoperative weeks (P<,0.001). Cortical and trabecular thicknesses, and femoral strength respectively and significantly decreased in the experimental group 1 .X=220.80&plusmn;5.90,P<0.001; X=90.34&plusmn;5.73,P=0.001; X=5.15&plusmn;1.07,P=0.002. In the experiment. group 2, decrease was .X=255.40&plusmn;6.02,P<0.001; X=113.50&plusmn;3.43, P=0.008; X=8.00&plusmn;1.11,P=0.015; respectively, comparing with the control group (X=232.36&plusmn;5.13, X=100.50&plusmn;5.06, X=6.95&plusmn;1.16). Cortical and trabecular thicknesses, and bone strength significantly increased in the experimental group 2, compared to the experimental&nbsp; group 1(P<0.001).&nbsp; There was also a significant decrease in the trabecular distance in the experimental group 2 (X=111.60&plusmn;2.87) compared to the experimental 1 (X=127.40&plusmn;4.74, P<0.001). Conclusion: Pulsed electromagnet, can be effective on the osteoporosis improvement.